Fundamentals of Light Microscopy and Electronic Imaging, Second Edition Douglas B. Murphy provides an extensive introduction to the principles and applications of light microscopy and electronic imaging. This textbook covers both the theoretical foundation and practical aspects of integrated optical microscope systems.
Description
This reference work discusses fundamental optical principles, basic forms of light microscopy, and modern electronic imaging systems. The content is expanded with topics such as multispectral imaging, enhanced digital cameras, and signal colocalization. New chapters address live-cell imaging, protein dynamics, deconvolution, and interferometric microscopy. Each chapter combines theory with practical guidance for instrument alignment and image interpretation.
The book supports instructors with additional materials, including PowerPoint slides of images. It is aimed at professionals and students who want to deepen their knowledge of microscopy and electronic imaging techniques.
Product specifications
- Author: Douglas B. Murphy (University of Pennsylvania, Howard Hughes Medical Institute)
- Publisher: John Wiley and Sons Ltd
- Imprint: Wiley-Blackwell
- Publication date: 2012-10-19
- Number of pages: 560
- ISBN: 9780471692140
- Subject: Microscopy
- BISAC: SCIENCE / Microscopes & Microscopy
About the author
DOUGLAS B. MURPHY leads microscopy and histology facilities at the HHMI Janelia Farm Research Campus and is an adjunct professor of cell biology at Johns Hopkins School of Medicine. He has extensive experience in setting up and managing microscope facilities.
MICHAEL W. DAVIDSON is affiliated with the National High Magnetic Field Laboratory and Florida State University, where he is dedicated to educational websites. His digital microscopic images have been published on countless front covers.

